Biolab

Protocols and instructions for common lab procedures

Download the following reference documents to support your work in the lab when research calls for these popular procedures, protocols, and equipment.

Lab report instructions

View or download these lab report instructions for guidance in formatting your lab report, properly using citations, and other tips for writing excelleting reports.


Equipment instructions

Using the TECAN Infinite M200 plate reader for a single absorbance reading.
Plate Reader

Initial set-up of the S.I. Photonics Spectrophotometer.
Spectrophotometer

Using the C6 flow cytometer.
Cytometer


Protocols and procedures

Download instructions for the following lab procedures and protocols.

Instructions and tips for making solutions


Calculating concentrations and performing dilutions


Guidelines for culturing cells without contamination

Instructions for using the BSA method to determine protein concentration


How to isolate peripheral mononuclear cells from whole blood


Instructions for tagging proteins using flow cytometry

Use polymerase chain reaction (Traditional PCR) to copy DNA sequences


Instructions for using agarose gel electrophoresis


Use RT-qPCR, a three-step procedure of RNA isolation, reverse transcription and PCR