Protocols and instructions for common lab procedures

Download the following reference documents to support your work in the lab when research calls for these popular procedures, protocols, and equipment.

Lab report instructions

View or download these lab report instructions for guidance in formatting your lab report, properly using citations, and other tips for writing excelling reports.

Equipment instructions

Using the TECAN Infinite M200 plate reader for a single absorbance reading.
Plate Reader

Initial set-up of the S.I. Photonics Spectrophotometer.

Using the C6 flow cytometer.

Protocols and procedures

Download instructions for the following lab procedures and protocols.

Instructions and tips for making solutions

Calculating concentrations and performing dilutions

Guidelines for culturing cells without contamination

Instructions for using the BSA method to determine protein concentration

How to isolate peripheral mononuclear cells from whole blood

Instructions for tagging proteins using flow cytometry

Use polymerase chain reaction (Traditional PCR) to copy DNA sequences

Instructions for using agarose gel electrophoresis

Use RT-qPCR, a three-step procedure of RNA isolation, reverse transcription and PCR